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Summary: Molecular Biology
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Read the summary and the most important questions on Molecular Biology
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1 Phylogenetics
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What do phylogenetic trees represent?
Evolutionary relationships between groups of organisms, or among a family of related molecular sequences. -
On what assumption relies phylogenetics?
That all organisms are descended from a common ancestor -
2 DNA analysis
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What is the function of PCR buffer in the PCR reaction mixture?
Maintains correct pH during the reaction -
What is the function of MgCl2 in the PCR reaction mixture?
Provides Mg2+ ions, essential co-factor for the Taq polymerase -
What is the function of dNTPs in the PCR reaction mixture?
Substrate for newly synthesised DNA -
What is the function of forward/reverse primer in the PCR reaction mixture?
Allows initiation of forward/reverse strand DNA synthesis at a specific site -
What is the function of Taq DNA polymerase in the PCR reaction mixture?
Catalyses DNA synthesis; Taq polymerase is functional at the high temperatures required to denature dsDNA -
What is the function of DNA in the PCR reaction mixture?
Contains target template sequence for initial rounds of amplification -
What is the function of water in the PCR reaction mixture?
Used to adjust all components to correct final concentrations -
What happens at 94 degrees in the PCR reaction?
Denaturation, the two strands of DNA become separated
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